Litesizer™ 500
Particle analysis at the touch of a button
The Litesizer™ 500 is an instrument for characterizing nano- and microparticles in dispersions and solutions. It determines particle size, zeta potential, and molecular mass by measuring dynamic light scattering (DLS), electrophoretic light scattering (ELS), and static light scattering (SLS).
Moreover, the Litesizer™ 500 is the only DLS-based particle analyzer that is able to perform a straightforward measurement of the sample’s refractive index.
A highlight of the Litesizer™ 500 is its ingeniously simple software. We have created a one-page workflow, where input parameters, results and analysis are all on a single page: You can set up your experiments in a few seconds, and produce the analyses and reports you need at the touch of a button.
Key Features
Continuous transmittance measured automatically
Transmittance gives you instant feedback about your sample, and allows automatic optimization of measurement parameters (angle, focus position, and measurement duration).
Accurate determination of the refractive index
While for most particle analyzers these indices must be determined from external sources, the Litesizer™ 500 is able to measure the refractive index for the exact wavelength and temperature of your experiment. This ensures maximum accuracy of particle size and zeta potential results under all experimental conditions.
The Litesizer™ 500 determines the solvent refractive index within ±0.5 %, as defined by ISO 22412:2017 concerning the accuracy of the refractive index required for DLS.
Particle-size measurements: Unprecedented resolution
Several different particle sizes in a single suspension can be precisely resolved, thanks to highly developed algorithms in the firmware.
cmPALS – new patented technology for zeta potential
With cmPALS (European Patent 2 735 870), measurements can be performed at much lower applied electric fields, and within significantly shorter measuring times, thereby enabling reliable measurements of even highly sensitive samples.
Unique Ω-shaped capillary tube for zeta potential
Measurements are more stable and reproducible because of the unique Ω-shaped capillary in the cuvette.
Ingeniously simple software, Kalliope™
Kalliope™ provides an intuitive one-page workflow, where input parameters, measurements and analyses are all at hand. Kalliope™ is fully compliant with the FDA’s 21 CFR Part 11 regulations.
Technical Specifications
| General specifications | |||
|---|---|---|---|
| Temp. control range | 0–90 °C | ||
| Light source | Laser / 40 mW, 658 nm | ||
| Laser warm-up time | 6 min | ||
| Ambient operating temp. | 10–35 °C | ||
| Humidity | up to 90 % non-condensing | ||
| Dimensions (W, D, H) | 460 mm, 485 mm, 135 mm | ||
| Weight | approx. 18 kg (40 lb) | ||
| Particle size specifications | |||
| Measuring range | 0.3 nm – 10 μm* (particle diameter) | ||
| Sensitivity | |||
| min. conc. (protein) | 0.1 mg/mL (lysozyme) | ||
| min. conc. (polymer) | 1 µg/mL (polystyrene latex) | ||
| Accuracy | Better than +/-2 % on certified reference material | ||
| Repeatability | Better than +/-2 % on certified reference material | ||
| Minimum sample volume | 12 µL | ||
| Measurement angles | 15°, 90°, 175° | ||
| Zeta potential specifications | |||
| Measuring range | -600 to +600 mV | ||
| Mobility | 10-11 m²/V.s to 2 x 10-7 m²/V.s | ||
| Size range | 3.8 nm – 100 µm | ||
| Sensitivity | 1 mg/mL (lysozyme) | ||
| Maximum sample concentration | 40 % w/v | ||
| Sample volume | 350 µL | ||
| Maximum sample conductivity | 200 mS/cm | ||
| Molecular mass specifications | |||
| Measuring range (mass) | 980 Da – 20 MDa | ||
| Measuring range (particle size) | up to 40 nm (diameter) | ||
| Sensitivity | 0.1 mg/mL (lysozyme) | ||
| Accuracy | +/-10 % | ||
| Repeatability | +/-5 % | ||
| Measurement angle | 90° | ||
| Transmittance specifications | |||
| Size range | No limit | ||
| Measuring time | 10 s | ||
| Minimum sample volume | 15 µL | ||
| Refractive index specifications | |||
| Measuring range | 1.28 to 1.50 | ||
| Accuracy | +/-0.5 % | ||
| Temperature range | 0 °C to 90 °C | ||
| Wavelength | 658 nm | ||
| Minimum sample volume | 1 mL | ||
| Wetted parts: | Quartz, glass | ||
* under laboratory conditions
Standards
A
ASTM D4001-13ASTM E2490-09
ASTM E2865-12
I
ISO 13099-2ISO 13321
ISO 22412
Documents
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Faster, More Sensitive Zeta-Potential Measurements with cmPALS and the Litesizer™ 500 Application Reports de, en
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Handle With Care: Measuring Zeta Potential on High Conductivity Protein Samples Application Reports en
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Printing Ink: Gauging the Viscosity to Fine-Tune the Particle Size Calculation Application Reports en
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Simultaneous Resolution of Three Particle Sizes in a Single Suspension by Using DLS Application Reports de, en
