Protein Mass Determination using Small Angle X-Ray Scattering
The molecular weight of glucose isomerase was determined with small-angle X-ray scattering. The results are in good agreement with calculated values from X-ray crystallography data.
Protein mass determination by SAXS
Small-angle X-ray scattering (SAXS) is a nondestructive and quick method to study the structure of proteins in solution, i.e. in their native state. The mass of protein particles in solution is an important parameter giving quickly information about the aggregation state of the sample. It therefore can be used as a quality control parameter as it readily identifies undesired denaturation and aggregation of the proteins present in the sample.
Moreover, SAXS is able to identify the degree of oligomerization of proteins in its native environment. Even if the high resolution structure is known from protein crystallography it is not always clear, if it exists as a monomer or an oligomer in its native state.
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