Density as a tool for monitoring E. coli cell integrity
Using the density of culture supernatant as an at-line monitoring tool offers a straightforward and efficient method for assessing cell integrity in biotechnological processes. A university study followed this approach for monitoring E. coli cell integrity during recombinant protein production.
The affordability of a DMA density meter coupled with its growing use in bioscience and the pharmaceutical industry make it a practical addition to existing monitoring technologies, facilitating data collection for established processes.
Once incorporated into existing bioprocess monitoring systems density measurements offer several benefits:
- Short analysis time (a requirement of PAT tools for monitoring): Density measurements can be obtained quickly, allowing for prompt detection of cell integrity issues.
- Low analytical error: The simplicity of the method reduces the risk of operator errors.
- Cost-effectiveness: Measuring density is inexpensive compared to more complex analytical techniques.
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