White paper: Lactose analysis in milk using a density and sound velocity meter
This paper evaluates the use of an Anton Paar Density and Sound Velocity Meter for lactose quantification in milk via enzymatic hydrolysis. The results were compared to a high-resolution ultrasonic (HR-US) spectroscopy reference method and showed strong agreement. The use of density and sound velocity allows for reliable lactose determinations and opens up opportunities for broader applications in complex mixtures.
A novel ultrasonic method for precise quantification of lactose was recently developed by the research group of Prof. V. Buckin. This technique utilizes high-precision, real-time ultrasonic monitoring of lactose hydrolysis in milk by the enzyme β-galactosidase, enabling selective and accurate quantification of lactose content.
This method was adapted for use with an Anton Paar DMA 5001 Density and Sound Velocity Meter (DMA 5001 SV) and compared with the original method.
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