Characterization of Vaccine Delivery Systems using DLS and Zeta Potential Measurements

Characterization of Vaccine Delivery Systems using DLS and Zeta Potential Measurements


Vaccines have been playing an important role in advancing the human health for centuries and their rapid development have led to search for more immune dominant and protective antigens such as nano/microparticles to be used as subunit vaccines. Modulation of these subunit particle attributes such as size and charge can significantly impact the immune system by affecting their trafficking and/or engulfment. Among different techniques used to assess these attributes, light scattering approaches is one of the most commonly used approach as it offers a quick, easy and non-invasive method for these measurements. On one hand, Dynamic Light Scattering (DLS) measures the average size and polydispersity index of nanoparticle emulsions; the Electrophoretic Light Scattering (ELS) assesses the zeta potential measurement to govern their stability.

In this webinar we will be discussing the basics of Dynamic light scattering (DLS) and Electrophoretic light scattering (ELS) approaches for the determination of particle size and surface charge, respectively. Besides, glimpses will also be shared about the data interpretation and validity of methods for these techniques and their use in vaccine development and other pharmaceutical applications.

Key Takeaways:
- Importance of particle attributes in vaccine development 
- Dynamic light scattering technique for determination of particle size
- Zeta potential determination for assessing the stability 
- Litesizer 500 and its pharma-compliance Kalliope software
- Litesizer 500- Pharmaceutical applications

Language: English
Trainer: Dr. Rishi Gupta
Rishi Gupta

Dr. Rishi Gupta has done his Doctorate and Post-doctorate from University of Delhi. He has more than 12 years of experience in the field of catalytic reactions and analytical instrumentations, and has more than 40 publications to his credit. Dr. Gupta has rich experience in material characterisation and he has conducted several training programs on the related topics. Currently he is working as an Application Specialist in the Characterisation Division of Anton Paar India.


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