Characterization of Vaccine Delivery Systems using DLS and Zeta Potential Measurements
Vaccines have been playing an important role in advancing the human health for centuries and their rapid development have led to search for more immune dominant and protective antigens such as nano/microparticles to be used as subunit vaccines. Modulation of these subunit particle attributes such as size and charge can significantly impact the immune system by affecting their trafficking and/or engulfment. Among different techniques used to assess these attributes, light scattering approaches is one of the most commonly used approach as it offers a quick, easy and non-invasive method for these measurements. On one hand, Dynamic Light Scattering (DLS) measures the average size and polydispersity index of nanoparticle emulsions; the Electrophoretic Light Scattering (ELS) assesses the zeta potential measurement to govern their stability.
In this webinar we will be discussing the basics of Dynamic light scattering (DLS) and Electrophoretic light scattering (ELS) approaches for the determination of particle size and surface charge, respectively. Besides, glimpses will also be shared about the data interpretation and validity of methods for these techniques and their use in vaccine development and other pharmaceutical applications.
- Importance of particle attributes in vaccine development
- Dynamic light scattering technique for determination of particle size
- Zeta potential determination for assessing the stability
- Litesizer 500 and its pharma-compliance Kalliope software
- Litesizer 500- Pharmaceutical applications
Dr. Gupta earned his doctorate and post-doctoral degrees from the University of Delhi, and he is currently employed as Technical Manager with Anton Paar India's Characterization Division in Gurugram, Haryana. He has over 15 years of expertise working with catalytic reactions and analytical instruments. Dr. Gupta has over 40 international papers to his name, with around 3000 citations. Dr. Gupta also has extensive experience in porous material characterization and catalyst efficiency evaluation utilizing Gas Sorption Techniques and has led various training programs on the subject.
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