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Particle size analyzer:
Litesizer 500

Particle analysis at the touch of a button

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SKU: 155761
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The Litesizer 500 is an instrument for characterizing nano- and microparticles in dispersions and solutions. It determines particle size, zeta potential, and molecular mass by measuring dynamic light scattering (DLS), electrophoretic light scattering (ELS), and static light scattering (SLS).
Moreover, the Litesizer 500 is the only DLS-based particle analyzer that is able to perform a straightforward measurement of the sample’s refractive index.
A highlight of the Litesizer 500 is its ingeniously simple software. We have created a one-page workflow, where input parameters, results and analysis are all on a single page: You can set up your experiments in a few seconds, and produce the analyses and reports you need at the touch of a button.
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Key features

Continuous transmittance measured automatically

Transmittance gives you instant feedback about your sample, and allows automatic optimization of measurement parameters (angle, focus position, and measurement duration).

Accurate determination of the refractive index

While for most particle analyzers these indices must be determined from external sources, the Litesizer 500 is able to measure the refractive index for the exact wavelength and temperature of your experiment. This ensures maximum accuracy of particle size and zeta potential results under all experimental conditions.

The Litesizer 500 determines the solvent refractive index within ±0.5 %, as defined by ISO 22412:2017 concerning the accuracy of the refractive index required for DLS. 

Particle-size measurements: Unprecedented resolution

Several different particle sizes in a single suspension can be precisely resolved, thanks to highly developed algorithms in the firmware.

cmPALS – new patented technology for zeta potential

With cmPALS (European Patent 2 735 870), measurements can be performed at much lower applied electric fields, and within significantly shorter measuring times, thereby enabling reliable measurements of even highly sensitive samples.

Unique Ω-shaped capillary tube for zeta potential

Measurements are more stable and reproducible because of the unique Ω-shaped capillary in the cuvette.

Ingeniously simple software, Kalliope™

Kalliope™ provides an intuitive one-page workflow, where input parameters, measurements and analyses are all at hand. Kalliope™ is fully compliant with the FDA’s 21 CFR Part 11 regulations.

Technical specifications

General specifications
Temp. control range 0 °C–90 °C
Light source Semiconductor laser / 40 mW, 658 nm
Laser warm-up time 6 min
Ambient operating temp. 10 °C–35 °C
Dimensions (W, D, H) 460 mm, 485 mm, 135 mm
Weight approx. 18 kg (40 lb)
Particle size specifications
Measuring range 0.3 nm–10 μm* (particle diameter)
Minimum sample volume 12 µL
Sensitivity
Min. concentration 0.1 mg/mL (lysozyme)
Max. concentration 50 %m/v (sample-dependent)
Accuracy Better than +/-2 % on NIST traceable standards
Repeatability Better than +/-2 % on NIST traceable standards
Measurement angles 15°, 90°, 175°
Zeta potential specifications
Measuring range >= +/- 1000 mV
Mobility range 10 -11 m²/V.s to 2 x 10 -7 m²/V.s
Size range 3.8 nm–100 µm
Min. sample volume 50 µL (sample viscosity dependent)
Sensitivity 0.1 mg/mL (lysozyme)
Maximum sample concentration 70 %w/v
Accuracy +/- 10 %
Repeatability +/- 3 %
Maximum sample conductivity 200 mS/cm
Molecular mass specifications
Measuring range (mass) 980 Da–20 MDa
Measuring range (particle size) up to 40 nm (diameter)
Lowest concentration limit 0.1 mg/mL (lysozyme)
Accuracy +/-10 %
Repeatability +/-5 %
Measurement angle 90°
Transmittance specifications
Accuracy Better than +/-1 %
Measuring time 10 s
Minimum sample volume 15 µL
Refractive index specifications
Measuring range 1.28 to 1.50
Accuracy +/-0.5 %
Minimum sample volume 1 mL

*under laboratory conditions

Standards

A

ASTM D4001-13
ASTM E2490-09
ASTM E2865-12

I

ISO 13099-2
ISO 22412

U

USP 729

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