Recommended Results

Combined Dynamic Light Scattering and Refractive Index Measurement


Performing accurate dynamic light scattering (DLS) and electrophoretic light scattering (ELS) on particles in solution requires prior knowledge of the solvent’s refractive index. This dimensionless number describes how light propagates through a substance – or how much light is bent (refracted) when entering a material.

Although the refractive index of many solvents has been previously measured and published, it is important to note that the refractive index is strongly dependent on light frequency and, to a lesser extent, on temperature. Furthermore, the presence of particles in solution might modify the overall refractive index of the solvent. Plainly put, for some experiments, using ‘standard’ refractive index values instead of actual ones might skew the measurement results. 

The Litesizer™ 500 is the only DLS-based instrument on the market that enables you to perform a simple, straightforward measurement of the solvent refractive index using the same experimental set up as the subsequent scattering experiment. The refractive index results are directly incorporated into the DLS and ELS experiment by the software and do not require any complex data processing.

The solvent refractive index can now be accurately measured at the exact wavelength and temperature of the DLS or ELS experiment, ensuring maximum accuracy for particle size and zeta potential results under all experimental conditions. 

Date: 2017-07-11, 13:00 - 13:30
Language: English
Trainer: Vinod Radhakrishnan

Dr. Vinod Radhakrishnan is the Product Specialist for the Material Characterization division at Anton Paar USA. Dr. Radhakrishnan received his degrees in Chemical Engineering from Texas A&M University (MS) and Auburn University (Ph.D).

Contact person and registration:
Hayley Gilbert
Tel.: +1 - 804 - 550 - 1051[at]